"VV Square"building, Plot.No.TS 710/1b1 & 2B1, CMC Ward No 18, Moka road, Gandhinagar, Ballari-583 101. 583101 Bellari IN
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9788121932004- 5bb336ca20e0484bf194f531 PRACTICAL BOOK OF BIOTECH. & PLANT TISS https://www.trendypaper.com/s/5b1a00c581a9afd8ff765190/ms.products/5bb336ca20e0484bf194f531/images/5bb336ca20e0484bf194f532/5bb336ce20e0484bf194f54f/5bb336ce20e0484bf194f54f.jpeg

The book starts with an introduction to basic knowledge of instruments which deals with principle, working, uses, limitations and precautions of about ten instruments.Basic Knowledge of precaution of;Culture Media for Bacterial Growth, Plant Tissue Culture and Standard Solutions has been given in simple and easytofollow language. The biotechnology exercises such as Plasmid and DNA isolation, DNA size determination, Restriction digestion, PCR, Gus gene assay, RFLP, RAPD, Isolation of bacteria by streak and Pour plate method, Growth characteristics of E.Coli by Plating and Turbidimetric method and the plant tissues culture exercises such as Cell suspension culture, Androgenesis, Somatic embryogenesis, Preparation of plantlet to greenhouse field, have been given in a student friendly manner. Matter for Vivavoce has also been included. Table Of Contents: 1.Principle, working and uses of instruments: Autoclave..2.Preparation of culture media3. Preparation of Standard Solution4. Isolation of bacteria by streak plate method5. Isolation of bacteria by pour plate method6. To determine the growth characteristics of E.Coli7. Growth charatersitics of E.coli using turbidimetric method8. Isolation of plasmid from E.coli9. Isolation of bacterial genomic DNA from E.coli10. To determine the DNA molecular size11. Purification of DNA12. To perform Bacteriophage titration13. To perform restriction digestion of DNA with enzyme14.To perform tranformation of the given bacterial population15. DNA amplification by Polymerase chain reaction16. Preparation of Plant Tissue culture media17. To isolate protoplast from the plant tissue18. Vialibilty testing of protoplast19. Demonstrate of Protoplast fusion using polyethylene glycol20. Demonstration of Callus culture techniques21. Initiation of callus from radical tip of Phaseolus Vulgaris22.Demonstration of Cell Suspension Culture23. Demonstration of Androgensis is Datura24. Demonstration os Somatic embryogenesis25. Organogensis26.Growth Inde

9788121932004-
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S. Chand Publishing Ltd
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PRACTICAL BOOK OF BIOTECH. & PLANT TISS

PRACTICAL BOOK OF BIOTECH. & PLANT TISS

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Author: Nagar Santosh ,? Adhav Madhavi

Brand: S. Chand Publishing Ltd

ISBN: 9788121932004-
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The book starts with an introduction to basic knowledge of instruments which deals with principle, working, uses, limitations and precautions of about ten instruments.Basic Knowledge of precaution of;Culture Media for Bacterial Growth, Plant Tissue Culture and Standard Solutions has been given in simple and easytofollow language. The biotechnology exercises such as Plasmid and DNA isolation, DNA size determination, Restriction digestion, PCR, Gus gene assay, RFLP, RAPD, Isolation of bacteria by streak and Pour plate method, Growth characteristics of E.Coli by Plating and Turbidimetric method and the plant tissues culture exercises such as Cell suspension culture, Androgenesis, Somatic embryogenesis, Preparation of plantlet to greenhouse field, have been given in a student friendly manner. Matter for Vivavoce has also been included. Table Of Contents: 1.Principle, working and uses of instruments: Autoclave..2.Preparation of culture media3. Preparation of Standard Solution4. Isolation of bacteria by streak plate method5. Isolation of bacteria by pour plate method6. To determine the growth characteristics of E.Coli7. Growth charatersitics of E.coli using turbidimetric method8. Isolation of plasmid from E.coli9. Isolation of bacterial genomic DNA from E.coli10. To determine the DNA molecular size11. Purification of DNA12. To perform Bacteriophage titration13. To perform restriction digestion of DNA with enzyme14.To perform tranformation of the given bacterial population15. DNA amplification by Polymerase chain reaction16. Preparation of Plant Tissue culture media17. To isolate protoplast from the plant tissue18. Vialibilty testing of protoplast19. Demonstrate of Protoplast fusion using polyethylene glycol20. Demonstration of Callus culture techniques21. Initiation of callus from radical tip of Phaseolus Vulgaris22.Demonstration of Cell Suspension Culture23. Demonstration of Androgensis is Datura24. Demonstration os Somatic embryogenesis25. Organogensis26.Growth Inde

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